The content of ATP Synthase in Cerebral Ischemia of Varying Severity Comparative Analysis

Objective. Evaluation of changes in the content ATP synthase parietal cortex and hippocampus brain rats with ischemia varying severity a comparative aspect. Methods. The experiments were performed on 88 male outbred white weighing 260 ± 20 g. Brain was modeled under conditions intravenous thiopental anesthesia (40-50 mg / kg). Total cerebral by decapitation animals. sampling carried out 1 hour 24 hours after - to study tissue respiration mitochondria, as well later determine synthase. Subtotal simultaneous ligation both common carotid arteries. material taken Stepwise subtotal sequential arteries an interval 7 days. second artery each subgroups. Partial one right. operation. Determination immunohistochemical method using monoclonal antibodies. For this purpose, decapitation, quickly removed from rats, pieces fixed zinc-ethanol-formaldehyde at + 4 ° C (overnight), then embeddedвinвparaffin. Results. In group stepwise ischemia, smallest decrease observed 1st subgroup between dressings days, while greatest enzyme noted 3rd minimum (1 day). Modeling more severe types ischemic damage led pronounced morphological neurons rat their size, deformation perikarya, increase degree neuronal chromatophilia wrinkling subsequent death. These disorders most shortest dressings, which day, total ischemia. Conclusion. Thus, groups minimal time artery. suppression not so significant.